Location: LOC_COLKET-Colket Translational Research
Req ID: 223331
Shift: Days
Employment Status: Regular - Full Time
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The Lin and Xing laboratories at The Children's Hospital of Philadelphia and University of Pennsylvania seeks an experienced research technician to assist in running a busy experimental laboratory, including conducting routine molecular biology experiments. Among other duties, the candidate will have specific responsibilities in generating numerous genomic and transcriptomic sequencing libraries. In this position, the candidate will have the opportunity to work with state-of-the-art next-generation sequencing (NGS) technologies, including but not limited to RNA-seq, single-cell RNA-seq, Oxford Nanopore long-read sequencing, and others. The candidate will be involved in all aspects of the lab’s NGS workflows, developing new methodologies for these technologies, as well as implementing and troubleshooting NGS protocols.
The Lin and Xing laboratories study splicing in human diseases, including cancer. Post-transcriptional RNA processing and modifications are important mechanisms for gene regulation and functional diversity in eukaryotic cells. The lab develops and applies high-throughput sequencing strategies and transcriptomic engineering technologies to study the regulation and function of RNA splicing in human diseases.
The ideal candidate will:
Assist in running a busy experimental laboratory, including maintaining mammalian cell cultures, performing molecular assays including molecular cloning, and keeping clear experimental records according to a specific format. Communicate complex ideas and concepts to those with limited knowledge and understanding, as well as to peers. Have a Bachelor’s degree and at least 2 years of relevant experience or a Master’s degree in Molecular/Cell Biology, Biotechnology, or a related discipline from a leading academic institution. Have specific research expertise in the following areas are required or preferred: experience with handling RNA samples (required), NGS or third-generation sequencing experience(preferred), genomics studies(preferred).
- Independently performs advanced, complex research procedures on experimental samples
- Formulates and performs complex lab experiments and analyzes reports the data including interpretation of methodologies
- Performs advanced calculations and maintains appropriate log and lab notebooks
- Plans and modifies research protocols and procedures with minimal direction from the principal investigator or supervisor
- Assists in development & troubleshooting of new techniques and ideas
- Prepares laboratory reagents, monitors and maintains laboratory inventory
- Supervises, trains and mentors junior and new staff members
- Determines work assignments, schedules and laboratory priorities
- Presents & discusses work at weekly lab meetings and national symposia
- Assists in writing of grants, journal articles, poster and oral presentations
Required Education and Experience
Required Education: Bachelor’s Degree
Required Experience: Two (2) years of laboratory experience
Preferred Education, Experience & Cert/Lic
Preferred Education: Bachelor’s Degree
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CHOP also requires employees who work in patient care buildings to receive an annual influenza vaccine. Employees may request exemptions for valid religious and medical reasons. Start dates may be delayed until candidates are immunized or exemption requests are reviewed.
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